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KMID : 0606920140220040334
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Biomolecules & Therapeutics
2014 Volume.22 No. 4 p.334 ~ p.340
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Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana
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Li Wei
Yan Xi Tao
Sun Ya Nan
Ngan Thi Thanh
Shim Sang-Hee
Kim Young-Ho
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Abstract
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In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-κB inhibitory activity of the isolated compounds was measured in TNFα-treated HepG2 cells using a luciferase reporter system. Compounds 19?23 inhibited TNFα-stimulated NF-κB activation in a dose-dependent manner, with IC50 values ranging from 0.75?8.30 μM. Compounds 19 and 20 also inhibited the TNFα-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7?11 and 19?23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with EC50 values ranging from 0.9?10.8 μM. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.
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KEYWORD
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Pulsatilla koreana, NF-κB inhibitory activity, Tumor necrosis factor-α, PPAR
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